Files are available under licenses specified on their description page. A lectin from bothrops leucurus snake venom raises cytosolic. This work aimed at the isolation and functional characterization of a pla2 isolated from bothrops jararaca venom, named bjpla2i. Purification of a lectin with antibacterial activity from bothrops leucurus snake venom. The toxins of interest, a pi class metalloprotease mp and an acidic phospholipase a 2 pla 2, were isolated from b. Kinetic characterization of leucurobin, a coagulant thrombin.
Leucurogin, a new recombinant disintegrin cloned from bothrops leucurus. Joe pittman, from tampa, florida puts out an exceptional. For its purification, three consecutive chromatographic steps were used sephacryl s200, source 15q and mono q. Cytotoxic effect and apoptosis induction by bothrops leucurus venom lectin on tumor cell lines. Adult male wistar rats 260320 g were fasted for 24 h with free access to water. Bothrops leucurus mainly inhabits the northeast region of brazil, and its venom elicits biological effects that are typical of the bothrops genus, which include coagulative. Envenomation local features include the presence of fang marks, swelling. However, treatment with antivenom has limited effectiveness against venoms local effects. Inactivation and fragmentation of lectin from bothrops leucurus snake venom by gamma irradiation. A novel lectin was isolated from bothrops leucurus snake venom using a. In 2012, according to the brazilian ministry of health, around 28,000 cases of snake envenomations were reported, with the following predominance of genera responsible for accidents. Genus bothrops definition of genus bothrops by the free. Combining mitochondrial dna sequences and morphological data.
Venomous snakebite protocols from joe pittman thailandsnakes. Bothrops lanceolatus yellow viper granule seroyal usa disclaimer. The bothrops asper is the number one perpetrator of snake bite on human beings in its range or habitat. Leucurolysina leuca, a 23 kda nonhemorrhagic metalloproteinase, is found in venom of the viper bothrops leucurus. Aug, 2015 envenomation caused by snakes is a serious public health problem worldwide, especially in tropical and subtropical countries 14. We demonstrate fibrinolytic activity of leuca, in which the protease specifically degrades. Laminoacid oxidase from bothrops leucurus venom induces. Snake venom, bothrops jararaca, phospholipase a2, inflammation, cytotoxicity. Living hazards database search by scientific name pdf. Snake venom phospholipases a2 pla2s have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. Isolation and functional characterization of proin. The pit viper bothrops leucurus is the most common poisonous snake distributed throughout the remnant areas.
The pit viper bothrops leucurus is the most common poisonous snake distributed throughout the remnant areas of the brazilian atlantic forest between the states of maranhao and espirito santo and adapts well to densely populated urban areas 1. The pit viper bothrops leucurus whitetailedjararaca is a. Effect of bothrops leucurus venom in chick biventer. Venomous snakebite protocols from joe pittman i strongly suggest every one of you who handle venomous snakes invest in snakebite treatment protocols for the snakes you handle. Inability to articulate without any affection of the tongue, hemorrhages fluid and black. Jan 22, 2010 bothrops species snakebites account for the majority of envenomations in south and central america. Its cdnadeduced amino acid sequence reveals a multidomain structure including a proprotein, a metalloproteinase, a disintegrinlike and a cysteinerich domain. Tumor cytotoxicity of leucurolysinb, a piii snake venom metalloproteinase from bothrops leucurus gabriel lm 1, sanchez ef 2, silva sg 2, santos rg 1, 3 1 center for development of nuclear technologybrazilian nuclear energy commission cdtn cnen, belo horizonte. The pit viper bothrops leucurus whitetailedjararaca is a poisonous snake habitating areas in the northeast of brazil. There are no subspecies which are recognized as being valid. Kinetic characterization of leucurobin, a coagulant. One thing to be thankful for here, if youve forgotten to be thankful today, is that there arent any recognized sub species of this snake anywhere, but it is, of course, a southern cousin of the northern american pit vipers that weve all grown to know and not love.
This page was last edited on 6 december 2014, at 05. A lectin from bothrops leucurus snake venom raises. Cytotoxic and inflammatory potential of a phospholipase a 2. Bothrops is a genus of pit vipers endemic to central and south america. Bothrops jararacussu and bothrops jararaca venoms 10 mgml were preincubated with combretum leprosum extract 0. Purification of a lectin with antibacterial activity from bothrops. Bothrops leucurus wikispecies, free species directory.
Cytotoxic and inflammatory potential of a phospholipase a2 from. Another new and threatened species of lancehead genus bothrops serpentes, viperidae from ilha dos franceses, southeastern brazil. Bothrops pulchra achuarshiwiar makanch, aguaruna bakanch, jamunse, akawaio saroorooimo, scroorooimo, amahuaca shanoo, shanopan, aymara kollan catari, palli. All structured data from the file and property namespaces is available under the creative commons cc0 license. Here, we examine the biological consequences of leuca, including thrombolytic activity, direct effects on endothelial cells in culture and edematogenic activity in vivo. The pla 2 bleu txiii from bothrops leucurus snake venom was purified by reverse phase hplc according to method described by poncesoto et al. The generic name, bothrops, is derived from the greek words, bothros, meaning pit, and, ops, meaning eye or face, together an allusion to the heatsensitive loreal pit organs. Pdf hematological references for wild recentlycaught. These were assigned to bothrops megaera wagler, 1824 by 20, and then to b. Inactivation and fragmentation of lectin from bothrops.
Free disintegrins can be found in the snake venom as 510 kda hydrolysis. The journal publishes papers by leading scientists from all over the world that report on advances in the understanding of proteins in the broadest sense. L nh 4 hco 3 50 mm and centrifuged at 4500 g, and the supernatant was then applied on the analytical reverse. Bothrops synonyms, bothrops pronunciation, bothrops translation, english dictionary definition of bothrops. Acute renal failure is a common complication caused by bothrops viper envenomation. Approximately 2030 declared snakebite cases occurin martinique each year. The pit viper bothrops leucurus whitetailedjararaca is a poisonous snake whose natural habitat is the northeast of brazil. Find, read and cite all the research you need on researchgate. Pdf hematological references for wild recentlycaught white.
Snakebites are a public health problem, especially in tropical countries. Mar 27, 2019 the bothrops asper is the number one perpetrator of snake bite on human beings in its range or habitat. Cytotoxic and inflammatory potential of a phospholipase a. Original article reproductive and feeding biology of the. Bothrops ferdelance genus bothrops reptile genus a genus of reptiles crotalidae, family crotalidae new world vipers. Read cytotoxicity and inhibition of platelet aggregation caused by an l amino acid oxidase from bothrops leucurus venom, biochimica et biophysica acta bba general subjects on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. We added the venoms alone or the mixtures in a solution containing 0.
Abarema cochliacarpos extract decreases the inflammatory. Bothrops definition of bothrops by the free dictionary. This homeopathic product has not been evaluated by the food and drug administration for safety or efficacy. Swiss mice received perimuscular venom injection and were.
Leucurogin, a new recombinant disintegrin cloned from bothrops. This 56kda protein bears nlinked glycoconjugates and is a piii hemorrhagic metalloproteinase. Kinetic characterization of leucurobin, a coagulant thrombinlike enzyme from the venom of bothrops leucurus the open toxicology journal, 2010, 4. Bleu txiii was isolated from bothrops leucurus snake venom on onestep analytical chromatography reverse phase hplc, was homogeneous on sdspage, and was confirmed by qtof ultima api esims tof ms mode mass spectrometry in 14243. Bothrops leucurus, commonly known as the whitetail lancehead or the bahia lancehead, is a. Laaobl exhibited cytotoxicity, inducing apoptosis and necrosis in mdck and hk2.
Jul 01, 2011 read cytotoxicity and inhibition of platelet aggregation caused by an l amino acid oxidase from bothrops leucurus venom, biochimica et biophysica acta bba general subjects on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. In this study, the nefrotoxicity of a main component of b. Multiple alignments of bleu txiii show high degree of homology with basic pla 2 myotoxins from other bothrops venoms. Atroxlysiniii atriii was purified from the venom of bothrops atrox. Tumor cytotoxicity of leucurolysinb, a piii snake venom. The freeswimming medusae mainly stay in fairly shallow water max. Half moon bay, san mateo county, california october 2012 peter latourrette. Request pdf effect of bothrops leucurus venom in chick biventer cervicis preparation bothrops leucurus is a poorly studied pitviper found in northeastern brazil. Protein science, the flagship journal of the protein society, serves an international forum for publishing original reports on all scientific aspects of protein molecules. Pdf on dec 1, 2015, cristiana ferreira da silva and others published bothrops leucurus whitetail lancehead. Pdf purification of a lectin with antibacterial activity. Cytotoxic, thrombolytic and edematogenic activities of. Bothrops lanceolatus, a member of the crotalidae family, is considered to be the only involved snake.
The viper, bothrops leucurus, is a common venomous snake in northeastern brazil, including the states of bahia and espirito santo. Nov 23, 2018 snake venom phospholipases a2 pla2s have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. Media in category bothrops leucurus the following 4 files are in this category, out of 4 total. Its identity with bothropasin and jararhagin from bothrops. Bothrops definition is a genus of venomous pit vipers family crotalidae including the ferdelance b. Bothrops leucurus, commonly known as the whitetail lancehead or the bahia lancehead, is a species of venomous snake, a pit viper in the family viperidae. For its purification, three consecutive chromatographic steps were used. Sanchez, in handbook of proteolytic enzymes third edition, 20. Protein science aims to unify this field by cutting across. Amino acid sequence and crystal structure of bap1, a. Members of this genus are responsible for more human deaths in the americas than any other group of venomous.
Laaobl exhibited cytotoxicity, inducing apoptosis and necrosis in mdck and hk2 cell lines in a. The biological effects due to envenomation have a similar profile to those observed with other bothrops snakes, such as coagulant, hemorrhagic and fibrinolytic activity, as well as renal failure in vivo 8, 2. Morphological and molecular evidence for phylogeny and classification of south american pitvipers, genera bothrops, bothriopsis, and bothrocophias serpentes. Fda is not aware of scientific evidence to support homeopathy as effective. Here, we investigated the ability of abarema cochliacarpos hydroethanolic extract eac to protect mice against injection of bothrops leucurus venom. In both cases, no justification for separating these populations from either b. A profile of snake bites in brazil, to our study revealed that the association between male sex and poisoning severity increased from moderate to severe cases for victims of crotalus treated at specialized care centers. Molecular characterization of lys49 and asp49 phospholipases.531 27 362 422 1289 830 220 703 131 742 406 494 1052 1465 768 426 526 938 646 1087 1448 512 62 92 995 1476 1500 603 662 585 113 941 1412 1358 678 1289 1040 283 693 992 199 807 391